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h7 wicell wa07 (WiCell Research Institute Inc)

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WiCell Research Institute Inc h7 wicell wa07
H7 Wicell Wa07, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 96/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h7+wicell+wa07/WA07/pm41253931-41-9-10
Average 96 stars, based on 182 article reviews

h7 wicell wa07 - by Bioz Stars, 2026-09

96/100 stars

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Article Title: Optimization of gene knockout approaches and practical solutions to sgRNA selection challenges in hPSCs with inducible Cas9 system
Article Snippet: .. Two ES cell lines, H9 (Wicell, WA09) and H7 (Wicell, WA07), two iPSC cell lines, U2 [ ] and B1 [ ], were cultured in PGM1 (Pluripotency Growth Master 1) Medium (Cellapy, Beijing, China) on Matrigel (Corning, USA) pre-coated multi-well plates at 37°C with 5% CO 2 and passaged at 1:6 to 1:10 split ratio using 0.5 mM EDTA to dissociate cells when 80-90% cell confluency reached. .. Mycoplasma infection testing (Vazyme, Nanjing, China) was performed every other week to guarantee the cells were free of infection.

Article Title: Optimization of gene knockout approaches and sgRNA selection in hPSCs with inducible Cas9 expression
Article Snippet: .. Two Embryonic stem cell lines, H9 (Wicell, WA09) and H7 (Wicell, WA07), two iPSC cell lines, U2 and B1 , were cultured in PGM1 (Pluripotency Growth Master 1) Medium (Cellapy, Beijing, China) on Matrigel (Corning, USA) pre-coated multi-well plates at 37 °C with 5% CO 2 and passaged at 1:6 to 1:10 split ratio using 0.5 mM EDTA to dissociate cells when 80–90% cell confluency reached. .. hPSCs-iCas9 line was created by inserting doxcyline-spCas9-puromycin cassette into the AAVS1 (also known as PPP1R12C) locus. briefly, two Addgene vectors #73,500 and #113,194 were co-electroporated into hPSCs at 1:1 weight ratio using 4D-Nucelofector (Lonza Bioscience, Germany) at program CA137.

Article Title: Optimization of gene knockout approaches and sgRNA selection in hPSCs with inducible Cas9 expression.
Article Snippet: .. Two Embryonic stem cell lines, H9 (Wicell, WA09) and H7 (Wicell, WA07), two iPSC cell lines, U214 and B115, were cultured in PGM1 (Pluripotency Growth Master 1) Medium (Cellapy, Beijing, China) on Matrigel (Corning, USA) pre-coated multi-well plates at 37 °C with 5% CO2 and passaged at 1:6 to 1:10 split ratio using 0.5 mM EDTA to dissociate cells when 80–90% cell confluency reached. hPSCs-iCas9 line construction hPSCs-iCas9 line was created by inserting doxcyline-spCas9-puromycin cassette into the AAVS1 (also known as PPP1R12C) locus. briefly, two Addgene vectors #73,500 and #113,194 were co-electroporated into hPSCs at 1:1 weight ratio using 4D-Nucelofector (Lonza Bioscience, Germany) at program CA137. .. Vector #73,500 carries Tet-on® 3G system and provides spCas9/anti-puromycin cassette flanked by homology arms of the AAVS1 locus.

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